When you do this though, it might be best to chill the starter, and decant the mini beer off... since it will be oxidized from the reintroduction of alcohol. But if you put more o2 in the solution 6 hrs later, you will get more yeast growth.
Also, if you are pitching into a bigger beer/ super hoppy or roasty, or whatever, you probably don't have to worry about the 2 liters affecting the flavor profile of that big beer too much, so just pitch it. But if its a delicate blonde or something, chill and decant.
2¢
